Binh Diep, PhD

Professor

The Diep lab just received an IDIQ contract from the National Institutes of Allergy and Infectious Diseases for the “Pre-Clinical Models of Infectious Diseases – Task Area A – Small Animal Models of Infectious Diseases” program for the period from 2024 to 2031 (contract no. 75N93024D00018). As 1 of 33 awarded contractors under the PCMID program, the Diep lab is uniquely positioned to provide our six published rabbit infection models, including the acute bacterial skin and skin structure infection model, aortic valve endocarditis model, prosthetic joint infection model, non-ventilated pneumonia model, ventilator-associated pneumonia model, and hyperdynamic septic shock model, for preclinical efficacy testing of novel antimicrobial drugs, biologics and vaccine against both Gram-positive and Gram-negative bacteria.
Education
Postdoc, 2008 - Microbial Pathogenesis, University of California, San Francisco
PhD, 2005 - Infectious Diseases and Immunity, University of California, Berkeley
B.A., 2000 - Molecular Cell Biology, University of California, Berkeley
Honors and Awards
  • ICAAC Young Investigator Award, American Society for Microbiology, 2010
  • ASPIRE Young Investigator Award, Pfizer, 2009
Publications

  1. Gras E, Vu TTT, Nguyen NTQ, Tran VG, Mao Y, Tran ND, Mai NH, Dong OX, Jung DH, Iorio NLPP, Povoa HCC, Pinheiro MG, Aguiar-Alves F, Weiss WJ, Zheng B, Cheng LI, Stover CK, Sellman BR, DiGiandomenico A, Gibault L, Valour F, Diep BA. Development and validation of a rabbit model of Pseudomonas aeruginosa non-ventilated pneumonia for preclinical drug development. 2023. PMID: 38149013


  3. Aguiar-Alves F, Le HN, Tran VG, Gras E, Vu T, Dong O, Quetz JS, Cheng LI, Yu L, Sellman BR, Stover CK, DiGiandomenico A, Diep BA. Anti-virulence Bispecific Monoclonal Antibody Mediated Protection Against Pseudomonas aeruginosa Ventilator-Associated Pneumonia in a Rabbit Model. 2021. PMID: 34902264


  4. Mao Y, Valour F, Nguyen NTQ, Doan TMN, Koelkebeck H, Richardson C, Cheng LI, Sellman BR, Tkaczyk C, Diep BA. Multimechanistic Monoclonal Antibody Combination Targeting Key Staphylococcus aureus Virulence Determinants in a Rabbit Model of Prosthetic Joint Infection. 2021. PMID: 33903108


  5. Nguyen NTQ, Gras E, Tran ND, Nguyen NNY, Lam HTH, Weiss WJ, Doan TNM, Diep BA. Pseudomonas aeruginosa Ventilator-Associated Pneumonia Rabbit Model for Preclinical Drug Development. 2021. PMID: 33972247


  7. Schnurr E, Paqué PN, Attin T, Nanni P, Grossmann J, Holtfreter S, Bröker BM, Kohler C, Diep BA, Ribeiro AA, Thurnheer T. Staphylococcus aureus Interferes with Streptococci Spatial Distribution and with Protein Expression of Species within a Polymicrobial Oral Biofilm. 2021. PMID: 33530340


  8. Byrne JM, Waack U, Weinstein EA, Joshi A, Shurland SM, Iarikov D, Bulitta JB, Diep BA, Guina T, Hope WW, Lawrenz MB, Lepak AJ, Luna BM, Miesel L, Phipps AJ, Walsh TJ, Weiss W, Amini T, Farley JJ. FDA Public Workshop Summary: Advancing Animal Models for Antibacterial Drug Development. 2020. PMID: 33106262


  10. Tran VG, Venkatasubramaniam A, Adhikari RP, Krishnan S, Wang X, Le VTM, Le HN, Vu TTT, Schneider-Smith E, Aman MJ, Diep BA. Efficacy of Active Immunization with Attenuated Alpha-Hemolysin and Panton-Valentine Leukocidin in a Rabbit Model of Staphylococcus aureus Necrotizing Pneumonia. 2019. PMID: 31504652


  11. Leech JM, Dhariwala MO, Lowe MM, Chu K, Merana GR, Cornuot C, Weckel A, Ma JM, Leitner EG, Gonzalez JR, Vasquez KS, Diep BA, Scharschmidt TC. Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life Discrimination of Pathogenic versus Commensal Skin Bacteria. 2019. PMID: 31784259


  13. Le HN, Tran VG, Vu TTT, Gras E, Le VTM, Pinheiro MG, Aguiar-Alves F, Schneider-Smith E, Carter HC, Sellman BR, Stover CK, DiGiandomenico A, Diep BA. Treatment Efficacy of MEDI3902 in Pseudomonas aeruginosa blood stream infection and acute pneumonia rabbit models. 2019. PMID: 31160288


  14. Baude J, Bastien S, Gillet Y, Leblanc P, Itzek A, Tristan A, Bes M, Duguez S, Moreau K, Diep BA, Norrby-Teglund A, Henry T, Vandenesch F. Necrotizing Soft Tissue Infections S.aureus - but not S.pyogenes- isolates display high rate of internalization and cytotoxicity toward human myoblasts. 2019. PMID: 31001627


  15. J.M. Leech, K. Chu, E.G. Leitner, J.J. Moon, B.A. Diep, T.C. Scharschmidt. 925 Neonatal priming shapes preferential capacity for immune tolerance to skin commensal vs. pathogenic bacteria. 2018. PMID:


  16. Le HN, Quetz JS, Tran VG, Le VTM, Aguiar-Alves F, Pinheiro MG, Cheng L, Yu L, Sellman BR, Stover CK, DiGiandomenico A, Diep BA. MEDI3902 correlates of protection against severe Pseudomonas aeruginosa pneumonia in a rabbit acute pneumonia model. 2018. PMID: 29483116


  17. Gustave CA, Tristan A, Martins-Simões P, Stegger M, Benito Y, Andersen PS, Bes M, Le Hir T, Diep BA, Uhlemann AC, Glaser P, Laurent F, Wirth T, Vandenesch F. Demographic fluctuation of community-acquired antibiotic-resistant Staphylococcus aureus lineages: potential role of flimsy antibiotic exposure. 2018. PMID: 29599521


  18. Hodille E, Rose W, Diep BA, Goutelle S, Lina G, Dumitrescu O. The Role of Antibiotics in Modulating Virulence in Staphylococcus aureus. 2017. PMID: 28724662


  19. Le VT, Le HN, Pinheiro MG, Hahn KJ, Dinh ML, Larson KB, Flanagan SD, Badiou C, Lina G, Tkaczyk C, Sellman BR, Diep BA. Effects of Tedizolid Phosphate on Survival Outcomes and Suppression of Production of Staphylococcal Toxins in a Rabbit Model of Methicillin-Resistant Staphylococcus aureus Necrotizing Pneumonia. Volume 61 of Issue 4. 2017. PMID: 28137816


  20. Diep BA, Hilliard JJ, Le VT, Tkaczyk C, Le HN, Tran VG, Rao RL, Dip EC, Pereira-Franchi EP, Cha P, Jacobson S, Broome R, Cheng LI, Weiss W, Prokai L, Nguyen V, Stover CK, Sellman BR. Targeting Alpha Toxin To Mitigate Its Lethal Toxicity in Ferret and Rabbit Models of Staphylococcus aureus Necrotizing Pneumonia. Volume 61 of Issue 4. 2017. PMID: 28115346


  21. Hodille E, Cuerq C, Badiou C, Bienvenu F, Steghens JP, Cartier R, Bes M, Tristan A, Plesa A, Le VT, Diep BA, Lina G, Dumitrescu O. Delta Hemolysin and Phenol-Soluble Modulins, but Not Alpha Hemolysin or Panton-Valentine Leukocidin, Induce Mast Cell Activation. Volume 6. 2016. PMID: 28018862


  22. Le VT, Tkaczyk C, Chau S, Rao RL, Dip EC, Pereira-Franchi EP, Cheng L, Lee S, Koelkebeck H, Hilliard JJ, Yu XQ, Datta V, Nguyen V, Weiss W, Prokai L, O'Day T, Stover CK, Sellman BR, Diep BA. Critical Role of Alpha-Toxin and Protective Effects of Its Neutralization by a Human Antibody in Acute Bacterial Skin and Skin Structure Infections. Volume 60 of Issue 10. 2016. PMID: 27401576


  24. Diep BA, Le VT, Badiou C, Le HN, Pinheiro MG, Duong AH, Wang X, Dip EC, Aguiar-Alves F, Basuino L, Marbach H, Mai TT, Sarda MN, Kajikawa O, Matute-Bello G, Tkaczyk C, Rasigade JP, Sellman BR, Chambers HF, Lina G. IVIG-mediated protection against necrotizing pneumonia caused by MRSA. Volume 8 of Issue 357. 2016. PMID: 27655850


  25. Cohen TS, Hilliard JJ, Jones-Nelson O, Keller AE, O'Day T, Tkaczyk C, DiGiandomenico A, Hamilton M, Pelletier M, Wang Q, Diep BA, Le VT, Cheng L, Suzich J, Stover CK, Sellman BR. Staphylococcus aureus a toxin potentiates opportunistic bacterial lung infections. Volume 8 of Issue 329. 2016. PMID: 26962155


  26. Cheung GY, Yeh AJ, Kretschmer D, Duong AC, Tuffuor K, Fu CL, Joo HS, Diep BA, Li M, Nakamura Y, Nunez G, Peschel A, Otto M. Functional characteristics of the Staphylococcus aureus d-toxin allelic variant G10S. Volume 5. 2015. PMID: 26658455


  27. Chan LC, Basuino L, Diep B, Hamilton S, Chatterjee SS, Chambers HF. Ceftobiprole- and ceftaroline-resistant methicillin-resistant Staphylococcus aureus. Volume 59 of Issue 5. 2015. PMID: 25753637


  28. Jenkins A, Diep BA, Mai TT, Vo NH, Warrener P, Suzich J, Stover CK, Sellman BR. Differential expression and roles of Staphylococcus aureus virulence determinants during colonization and disease. Volume 6 of Issue 1. 2015. PMID: 25691592


  29. Date SV, Modrusan Z, Lawrence M, Morisaki JH, Toy K, Shah IM, Kim J, Park S, Xu M, Basuino L, Chan L, Zeitschel D, Chambers HF, Tan MW, Brown EJ, Diep BA, Hazenbos WL. Global gene expression of methicillin-resistant Staphylococcus aureus USA300 during human and mouse infection. Volume 209 of Issue 10. 2013. PMID: 24286981


  30. Diep BA, Phung Q, Date S, Arnott D, Bakalarski C, Xu M, Nakamura G, Swem DL, Alexander MK, Le HN, Mai TT, Tan MW, Brown EJ, Nishiyama M. Identifying potential therapeutic targets of methicillin-resistant Staphylococcus aureus through in vivo proteomic analysis. Volume 209 of Issue 10. 2013. PMID: 24280367


  31. Hazenbos WL, Kajihara KK, Vandlen R, Morisaki JH, Lehar SM, Kwakkenbos MJ, Beaumont T, Bakker AQ, Phung Q, Swem LR, Ramakrishnan S, Kim J, Xu M, Shah IM, Diep BA, Sai T, Sebrell A, Khalfin Y, Oh A, Koth C, Lin SJ, Lee BC, Strandh M, Koefoed K, Andersen PS, Spits H, Brown EJ, Tan MW, Mariathasan S. Novel staphylococcal glycosyltransferases SdgA and SdgB mediate immunogenicity and protection of virulence-associated cell wall proteins. Volume 9 of Issue 10. 2013. PMID: 24130480


  32. Le VT, Diep BA. Selected insights from application of whole-genome sequencing for outbreak investigations. Volume 19 of Issue 5. 2013. PMID: 23856896


  33. Diep BA, Gillet Y, Etienne J, Lina G, Vandenesch F. Panton-Valentine leucocidin and pneumonia. Volume 13 of Issue 7. 2013. PMID: 23809217


  34. Rasigade JP, Trouillet-Assant S, Ferry T, Diep BA, Sapin A, Lhoste Y, Ranfaing J, Badiou C, Benito Y, Bes M, Couzon F, Tigaud S, Lina G, Etienne J, Vandenesch F, Laurent F. PSMs of hypervirulent Staphylococcus aureus act as intracellular toxins that kill infected osteoblasts. Volume 8 of Issue 5. 2013. PMID: 23690994


  35. Diep BA, Afasizheva A, Le HN, Kajikawa O, Matute-Bello G, Tkaczyk C, Sellman B, Badiou C, Lina G, Chambers HF. Effects of linezolid on suppressing in vivo production of staphylococcal toxins and improving survival outcomes in a rabbit model of methicillin-resistant Staphylococcus aureus necrotizing pneumonia. Volume 208 of Issue 1. 2013. PMID: 23532096


  36. Diep BA. Use of whole-genome sequencing for outbreak investigations. Volume 13 of Issue 2. 2012. PMID: 23158673


  37. Adams-Sapper S, Diep BA, Perdreau-Remington F, Riley LW. Clonal composition and community clustering of drug-susceptible and -resistant Escherichia coli isolates from bloodstream infections. Volume 57 of Issue 1. 2012. PMID: 23147723


  38. Tattevin P, Schwartz BS, Graber CJ, Volinski J, Bhukhen A, Bhukhen A, Mai TT, Vo NH, Dang DN, Phan TH, Basuino L, Perdreau-Remington F, Chambers HF, Diep BA. Concurrent epidemics of skin and soft tissue infection and bloodstream infection due to community-associated methicillin-resistant Staphylococcus aureus. Volume 55 of Issue 6. 2012. PMID: 22670044


  39. Diep BA, Equils O, Huang DB, Gladue R. Linezolid effects on bacterial toxin production and host immune response: review of the evidence. Volume 73 of Issue 3. 2012. PMID: 24648596


  40. Li M, Du X, Villaruz AE, Diep BA, Wang D, Song Y, Tian Y, Hu J, Yu F, Lu Y, Otto M. MRSA epidemic linked to a quickly spreading colonization and virulence determinant. Volume 18 of Issue 5. 2012. PMID: 22522561


  41. Adams-Sapper S, Sergeevna-Selezneva J, Tartof S, Raphael E, Diep BA, Perdreau-Remington F, Riley LW. Globally dispersed mobile drug-resistance genes in gram-negative bacterial isolates from patients with bloodstream infections in a US urban general hospital. 2012. PMID: 22493279


  42. Perret M, Badiou C, Lina G, Burbaud S, Benito Y, Bes M, Cottin V, Couzon F, Juruj C, Dauwalder O, Goutagny N, Diep BA, Vandenesch F, Henry T. Cross-talk between Staphylococcus aureus leukocidins-intoxicated macrophages and lung epithelial cells triggers chemokine secretion in an inflammasome-dependent manner. Volume 14 of Issue 7. 2012. PMID: 22329718


  43. Malachowa N, Whitney AR, Kobayashi SD, Sturdevant DE, Kennedy AD, Braughton KR, Shabb DW, Diep BA, Chambers HF, Otto M, DeLeo FR. Global changes in Staphylococcus aureus gene expression in human blood. Volume 6 of Issue 4. 2011. PMID: 21525981


  44. Binh An Diep, George F. Sensabaugh, Naraporn Somboonna, Heather A. Carleton, Françoise Perdreau-Remington. Widespread Skin and Soft-Tissue Infections Due to Two Methicillin-Resistant Staphylococcus aureus Strains Harboring the Genes for Panton-Valentine Leucocidin. 2010. PMID:


  45. Diep BA, Chan L, Tattevin P, Kajikawa O, Martin TR, Basuino L, Mai TT, Marbach H, Braughton KR, Whitney AR, Gardner DJ, Fan X, Tseng CW, Liu GY, Badiou C, Etienne J, Lina G, Matthay MA, DeLeo FR, Chambers HF. Polymorphonuclear leukocytes mediate Staphylococcus aureus Panton-Valentine leukocidin-induced lung inflammation and injury. Volume 107 of Issue 12. 2010. PMID: 20231457


  46. Graves SF, Kobayashi SD, Braughton KR, Diep BA, Chambers HF, Otto M, Deleo FR. Relative contribution of Panton-Valentine leukocidin to PMN plasma membrane permeability and lysis caused by USA300 and USA400 culture supernatants. Volume 12 of Issue 6. 2010. PMID: 20172045


  47. Mendes RE, Sader HS, Deshpande LM, Diep BA, Chambers HF, Jones RN. Characterization of baseline methicillin-resistant Staphylococcus aureus isolates recovered from phase IV clinical trial for linezolid. Volume 48 of Issue 2. 2009. PMID: 19940054


  49. Miragaia M, de Lencastre H, Perdreau-Remington F, Chambers HF, Higashi J, Sullam PM, Lin J, Wong KI, King KA, Otto M, Sensabaugh GF, Diep BA. Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis. Volume 4 of Issue 11. 2009. PMID: 19893740


  50. Tseng CW, Kyme P, Low J, Rocha MA, Alsabeh R, Miller LG, Otto M, Arditi M, Diep BA, Nizet V, Doherty TM, Beenhouwer DO, Liu GY. Staphylococcus aureus Panton-Valentine leukocidin contributes to inflammation and muscle tissue injury. Volume 4 of Issue 7. 2009. PMID: 19633710


  51. Tattevin P, Diep BA, Jula M, Perdreau-Remington F. Methicillin-resistant Staphylococcus aureus USA300 clone in long-term care facility. Volume 15 of Issue 6. 2009. PMID: 19523301


  52. Schwartz BS, Graber CJ, Diep BA, Basuino L, Perdreau-Remington F, Chambers HF. Doxycycline, not minocycline, induces its own resistance in multidrug-resistant, community-associated methicillin-resistant Staphylococcus aureus clone USA300. Volume 48 of Issue 10. 2009. PMID: 19374563


  53. Li M, Diep BA, Villaruz AE, Braughton KR, Jiang X, DeLeo FR, Chambers HF, Lu Y, Otto M. Evolution of virulence in epidemic community-associated methicillin-resistant Staphylococcus aureus. Volume 106 of Issue 14. 2009. PMID: 19293374


  54. DeLeo FR, Diep BA, Otto M. Host defense and pathogenesis in Staphylococcus aureus infections. Volume 23 of Issue 1. 2009. PMID: 19135914


  55. Chambers HF, Basuino L, Diep BA, Steenbergen J, Zhang S, Tattevin P, Alder J. Relationship between susceptibility to daptomycin in vitro and activity in vivo in a rabbit model of aortic valve endocarditis. 2009. PMID: 19171803


  57. Graber CJ, Jacobson MA, Perdreau-Remington F, Chambers HF, Diep BA. Recurrence of skin and soft tissue infection caused by methicillin-resistant Staphylococcus aureus in a HIV primary care clinic. Volume 49 of Issue 2. 2008. PMID: 18820536


  58. Diep BA, Palazzolo-Ballance AM, Tattevin P, Basuino L, Braughton KR, Whitney AR, Chen L, Kreiswirth BN, Otto M, DeLeo FR, Chambers HF. Contribution of Panton-Valentine leukocidin in community-associated methicillin-resistant Staphylococcus aureus pathogenesis. Volume 3 of Issue 9. 2008. PMID: 18787708


  60. Diep BA, Otto M. The role of virulence determinants in community-associated MRSA pathogenesis. Volume 16 of Issue 8. 2008. PMID: 18585915


  61. Liu C, Graber CJ, Karr M, Diep BA, Basuino L, Schwartz BS, Enright MC, O'Hanlon SJ, Thomas JC, Perdreau-Remington F, Gordon S, Gunthorpe H, Jacobs R, Jensen P, Leoung G, Rumack JS, Chambers HF. A population-based study of the incidence and molecular epidemiology of methicillin-resistant Staphylococcus aureus disease in San Francisco, 2004-2005. Volume 46 of Issue 11. 2008. PMID: 18433335


  62. Diep BA, Stone GG, Basuino L, Graber CJ, Miller A, des Etages SA, Jones A, Palazzolo-Ballance AM, Perdreau-Remington F, Sensabaugh GF, DeLeo FR, Chambers HF. The arginine catabolic mobile element and staphylococcal chromosomal cassette mec linkage: convergence of virulence and resistance in the USA300 clone of methicillin-resistant Staphylococcus aureus. Volume 197 of Issue 11. 2008. PMID: 18700257


  64. Diep BA, Chambers HF, Graber CJ, Szumowski JD, Miller LG, Han LL, Chen JH, Lin F, Lin J, Phan TH, Carleton HA, McDougal LK, Tenover FC, Cohen DE, Mayer KH, Sensabaugh GF, Perdreau-Remington F. Emergence of multidrug-resistant, community-associated, methicillin-resistant Staphylococcus aureus clone USA300 in men who have sex with men. Volume 148 of Issue 4. 2008. PMID: 18283202


  65. Miller LG, Perdreau-Remington F, Bayer AS, Diep B, Tan N, Bharadwa K, Tsui J, Perlroth J, Shay A, Tagudar G, Ibebuogu U, Spellberg B. Clinical and epidemiologic characteristics cannot distinguish community-associated methicillin-resistant Staphylococcus aureus infection from methicillin-susceptible S. aureus infection: a prospective investigation. Volume 44 of Issue 4. 2007. PMID: 17243048


  66. Binh An Diep, George F. Sensabaugh, Francoise Perdreau-Remington. Reply to Seifried et al. 2007. PMID:


  67. Vivoni AM, Diep BA, de Gouveia Magalhães AC, Santos KR, Riley LW, Sensabaugh GF, Moreira BM. Clonal composition of Staphylococcus aureus isolates at a Brazilian university hospital: identification of international circulating lineages. Volume 44 of Issue 5. 2006. PMID: 16672394


  68. Diep BA, Carleton HA, Chang RF, Sensabaugh GF, Perdreau-Remington F. Roles of 34 virulence genes in the evolution of hospital- and community-associated strains of methicillin-resistant Staphylococcus aureus. Volume 193 of Issue 11. 2006. PMID: 16652276


  69. Diep BA, Gill SR, Chang RF, Phan TH, Chen JH, Davidson MG, Lin F, Lin J, Carleton HA, Mongodin EF, Sensabaugh GF, Perdreau-Remington F. Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus. Volume 367 of Issue 9512. 2006. PMID: 16517273


  70. Pan ES, Diep BA, Charlebois ED, Auerswald C, Carleton HA, Sensabaugh GF, Perdreau-Remington F. Population dynamics of nasal strains of methicillin-resistant Staphylococcus aureus--and their relation to community-associated disease activity. Volume 192 of Issue 5. 2005. PMID: 16088830


  71. Carleton HA, Diep BA, Charlebois ED, Sensabaugh GF, Perdreau-Remington F. Community-adapted methicillin-resistant Staphylococcus aureus (MRSA): population dynamics of an expanding community reservoir of MRSA. Volume 190 of Issue 10. 2004. PMID: 15499526


  72. Lagally ET, Scherer JR, Blazej RG, Toriello NM, Diep BA, Ramchandani M, Sensabaugh GF, Riley LW, Mathies RA. Integrated portable genetic analysis microsystem for pathogen/infectious disease detection. 2004. PMID: 15167797


  73. Charlebois ED, Perdreau-Remington F, Kreiswirth B, Bangsberg DR, Ciccarone D, Diep BA, Ng VL, Chansky K, Edlin BR, Edlin B, Chambers HF. Origins of community strains of methicillin-resistant Staphylococcus aureus. Volume 39 of Issue 1. 2004. PMID: 15206052


  74. Diep BA, Sensabaugh GF, Somboonna N, Somboona NS, Carleton HA, Perdreau-Remington F. Widespread skin and soft-tissue infections due to two methicillin-resistant Staphylococcus aureus strains harboring the genes for Panton-Valentine leucocidin. Volume 42 of Issue 5. 2004. PMID: 15131173


  75. Pan ES, Diep BA, Carleton HA, Charlebois ED, Sensabaugh GF, Haller BL, Perdreau-Remington F. Increasing prevalence of methicillin-resistant Staphylococcus aureus infection in California jails. Volume 37 of Issue 10. 2003. PMID: 14583874